EF

EF-1a (ABP50)

EF-1a is a pH-sensitive actin binding protein present in presumably all eukaryotic cells as it is pivotally involved in mRNA translation. EF-1a also binds microtubules and in some circumstances, severs them (Shiina et al, 1994). EF-1a can bind microfilaments (Moore, & Cyr, 2000) and microtubules (Itano & Hatano, 1991). The EF-1a gene has also been found to be important in deciphering phylogenetic relationships (Berberich et al, 1995; Roger et al, 1999; Gaucher et al, 2001).

References:-

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Ransom, W. D., Lao, P.-C., Gage, D. A. & Boss, W. F. (1998) Phosphoglycerylethanolamine posttranslational modification of plant eukaryotic elongation factor 1a., Plant Physiol. 117, 949-960.

Roger, A. J., Sandblom, O., Doolittle, W. F. & Philippe, H. (1999) An evaluation of elongation factor 1a as a phylogenetic marker for eukaryotes., J.Mol. Evol. 16, 218-233.

Reynet, C. & Kahn, C. R. (2001) Unbalanced expression of the different subunits of elongation factor 1 in diabetic skeletal muscle., PNAS. 98, 3422-3427.

Gaucher, E. A., Miyamoto, M. M. & Benner, S. A. (2001) Function-structure analysis of proteins using covarion-based evolutionary approaches: Elongation factors., PNAS. 98, 548-552.

Moore, R. C. & Cyr, R. J. (2000) Association between elongation factor-1a and microtubules in vivo is domain dependent and conditional., Cell Mot.Cytoskeleton. 45, 279-292.

Hayashi, Y., Urade, R., Utsumi, S. & Kito, M. (1989) Anchoring of peptide elongation factor EF-1a by phosphatidylinositol at the endplasmic reticulum membrane., J.Biochem. 106, 560-563.

Habben, J. E., Moro, G. L., Hunter, B. G., Hamaker, B. R. & Larkins, B. A. (1995) Elongation factor 1a concentration is highly correlated with the lysine content of maize endosperm., Proc.Nat.Acad. Sci. 92, 8640-8644.

Yang, F., Demma, M., Warren, V., Dharmawardhane, S. & Condeelis, J. (1990) Identification of an actin-binding protein from Dictyostelium as elongation factor 1a, Nature. 347, 494-496.

Edmonds, B. T., Murray, J. & Condeelis, J. (1995) pH regulation of the F-actin binding properties of Dictyostelium elongation factor 1a, J. Biol. Chem. 270, 15222-15230.

Itano, N. & Hatano, S. (1991) F-actin bundling protein from Physarum polycephalum: Purification and its capacity for co-bundling of actin filaments and microtubules., Cell Mot.Cytoskel. 19, 244-254.

Petrushenko, Z. M., Negrutskii, B. S., Ladokhin, A. S., Budkevich, T. V., Shalak, V. & El'skaya, A. V. (1997) Evidence for the formation of an unusual ternary complex of rabbit liver EF-1a with GDP and deacylated tRNA., FEBS letters. 407, 13-17.

Murray, J. W., Edmonds, B. T., Liu, G. & Condeelis, J. (1996) Bundling of actin filaments by elongation factor 1a inhibits polymerization at filaments ends., J.Cell Biol. 135, 1309-1321.

Condeelis, J. (1995) Elongation factor 1a, translation and the cytoskeleton, TIBS. 20, 169-170.

Kurasawa, Y., Hanyu, K., Watanabe, Y. & Numata, O. (1996) F-actin bundling activity of Tetrahymena elongation factor 1a is regulated by Ca²⁺/calmodulin., J.Biochem. 119, 791-798.

Liu, G., Tang, J., Edmonds, B. T., Murray, J., Levin, S. & Condeelis, J. (1996) F-actin sequesters elongation factor 1a from interaction with aminoacyl-tRNA in a pH-dependent reaction., J.Cell Biol. 135, 953-963.

Numata, O., Kurasawa, Y., Gonda, K. & Watanabe, Y. (2000) Tetrahymena elongation factor-1a is localized with calmodulin in the division furrow., J.Biochem. 127, 51-56.

Bektas, M., Nurten, R., Gürel, Z., Sayers, Z. & Bermek, E. (1994) Interactions of eukaryotic elaongation factor 2 with actin: a possible link bewteen protein synthetic machinery and cytoskeleton., FEBS letters. 356, 89-93.

Suda, M., Fukui, M., Sogabe, Y., Sato, K., Morimatsu, A., Arai, R., Motegi, F., Miyakawa, T., Mabuchi, I. & Hirata, D. (1999) Overproduction of elongation factor 1a, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast., Genes to Cells. 4, 517-527.

Carr-Schmid, A., Durko, N., Cavallius, J., Merrick, W. C. & Kinzy, T. G. (1999) Mutations in a GTP-binding motif of eukaryotic elongation factor 1a reduce both tranlational fidelity and the requirement for nucleotide exchange., J.Biol.Chem. 274, 30297-30302.

Demma, M., Warren, V., Hock, R., Dharmawardhane, S. & Condeelis, J. (1990) Isolation of an abundant 50,000-dalton actin filament bundling protein from Dictyostelium amoebae., J.Biol.Chem. 265, 2286-2291.

Kaur, K. J. & Ruben, L. (1994) Protein translation elongation factor-1a from Trypanosoma brucei binds calmodulin., J.Biol.Chem. 269, 23045-23050.

Bassel, G. J., Powers, C. M., Taneja, K. L. & Singer, R. H. (1994) Single mRNAs visualized by ultrastructural in situ hybridization are principlally localized at actin filament intersections in fibroblasts., J.Cell Biol. 126, 863-876.

Shiina, N., Gotoh, Y., Kobomura, N., Iwamatsu, A. & Nishida, E. (1994) Microtubule severing by elontation factor 1a, Science. 266, 282-285.

Kurasawa, Y., Numata, O., Katoh, M., Hirano, H., Chiba, J. & Watanabe, Y. (1992) Identifiaction of Tetrahymena 14-nm filament-associated protein as elongation factor 1a., Exp.Cell Res. 203, 251-258.

Edmonds, B. T., Wyckoff, J., Yeung, Y.-G., Wang, Y., Stanley, E. R., Jones, J., Segali, J. & Condeelis, J. (1996) Elongation factor -1a is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma., J.Cell Sci. 109, 2705-2714.

Durso, N. A. & Cyr, R. J. (1994) Beyond translation: elongation factor-1a and the cytoskeleton., Protoplasma. 180, 99-105.

Dharmawardhane, S., Demma, M., Yang, F. & Condeelis, J. (1991) Compartmentalization and actin binding properties of ABP-50: The elongation factor 1 alpha of Dictyostelium., Cell Mot.Cytoskel. 20, 279-288.

Edmonds, B. T. (1993) ABP50: An actin-binding elongation factor 1a from Dictyostelium discoideum., J.Cell.Biochem. 52, 134-139.

Yang, W., W., B., Cavallius, J., Merrick, W. C. & Boss, W. F. (1993) Purification and characterization of a phosphatidylinositol 4-kinase activator in carrot cells., J.Biol.Chem. 268, 392-398.

Durso, N. A. & Cyr, R. J. (1994) A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1a., The Plant Cell. 6, 893-905.

Zischka, H., Oehme, F., Pintsch, T., Ott, A., Keller, H., Kellermann, J. & Schuster, S. (1999) Rearrangment of cortex proteins constitutes an osmoprotective mechanism in Dictyostelium., EMBO J. 18, 4241-4249.

Moore, R. C., Durso, N. A. & Cyr, R. J. (1998) Elongation factor-1a stabilizes microtubules in a calcium/calmodulin-dependent manner., Cell Mot.Cytoskel. 41, 168-180.

Nakazawa, N., Moreira, D., Laurent, J., Le Guyader, H., Fukami, Y. & Ito, K. (1999) Biochemical analysis of the interaction between elongation factor 1a and a/b-tubulins from a ciliate, Tetrahymena pyriformis., FEBS letters. 453, 29-34.

Nielsen, P. S., Kleinhofs, A. & Olsen, O.-A. (1997) Barley elongation factor 1a : genomic organization, DNA sequence, and phylogenetic implications, Genome. 40, 559-565.

Berberich, T., Sugawara, K., Harada, M. & Kusano, T. (1995) Molecular cloning, characterization and expression of an elongation factor 1a gene in maize, Plant Mol.Biol. 29, 611-615.

Gungabissoon, R. A., Khan, S., Hussey, P. J. & Maciver, S. K. (2001) Interaction of EF1a from Zea mays (ZmEF1a) with F-actin and interplay with the maize actin severing protein, ZmADF3., Cell Motility Cytoskeleton. 49, 104-111.