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An
54-58kDa monomeric actin filament
bundling protein originally isolated from sea urchin egg but also found in Drosophila
(Bryan et al,
1993), vertebrates
(Holthius et
al, 1994;
Edwards & Bryan 1995)
including man (Yamashiro-
Matsumara & Matsumara, 1985). Fascin
(from the latin for
bundle) is spaced at 11nm intervals along the filament. The bundles in cross
section are seen to be hexagonally packed this and the longnitudinal spacing is
compatible with a model (Bryan
& Kane, 1978)
where fascin cross-links at alternating 4 and 5 actins. It does seem likely that it may be most closely related to the
fimbrin/plastin group since it is, like them, calcium insensitive, monomeric and
close in apparent molecular weight.
Fascin
binds b-catenin
(Tao et al,
1996), a molecule
that is not only part of cell-cell adhesion, but is also part of the wnt
signalling pathway. It has been proposed that fascin reduces the cytplasmic pool
of soluble b-catenin
and so would be expected to act as an anti-oncogene.
References:-
Bryan,
J., R. Edwards, et al. (1993). “Fascin,
an echinoid actin-bundling protein, is a homolog of the Drosophila singed
gene product.” Proc.Natl.Acad.Sci.USA
90, 9115-9119.
Bryan,
J. & Kane, R.E (1978). "" J.Mol.Biol. 125, 207-224.
Cant,
K., Knowles, B.A., Mooseker, M.S. & Cooley, L. (1994).
“Drosophila singed, a
fascin homolog, is required for actin bundle formation during oogenesis and
bristle extension.” J.Cell
Biol. 125(2), 369-380.
Dickinson,
W. J. and J. W. Thatcher (1997). “Morphogenesis of denticles and hairs in Drosophila
embryos: Involvement of actin-associated proteins that also affect adult
structures.” Cell Mot.Cytoskel. 38, 9-21.
Edwards,
R. A., H. Herrera-Sosa, et al. (1995). “Cloning and expression of a murine
fascin homolog from mouse brain.” J. Biol. Chem. 270(18),
10764-10770.
Edwards,
R. A. and J. Bryan (1995). “Fascins, a family of actin bundling proteins.” Cell
Mot. and the Cytoskel. 32, 1-9.
Holthuis, J. C.
M., V. T. G. Schoonderwoert, et al. (1994). “A vertebrate homolog of the
actin-bundling protein fascin.” Biochim.Biophys.Acta. 1219,
184-188.
Jawhari, A. U., Buda, A., Jenkins, M., Shehzad,
K., Sarraf, C., Noda, M., Farthing, M. J. G., Pignatelli, M. & Adams, J. C.
(2003) Fascin, an actin-bundling protein , modulates colonic epithelial cell
invasiveness and differentiation in vitro., Am J Pathol. 162,
69-80.
Tao, Y.S., Edwards, R.A., Tubb, B., Wang, S.
Bryan, J. & McCrea, P.D. (1996). "" J.Cell Biol. 134,
1271-1281.
Tilney,
L. G., M. S. Tilney, M.S. & Guild, G.M. (1995).
“F actin bundles in Drosophila bristles I. Two filament cross-links are
involved in bundling.” J.Cell Biol. 130(3), 629-638.
Yamashiro-Matsumara, S.
& Matsumara, F.(1985). "" J.Biol.Chem. 260,
5087-5097.
Yamashiro, S.,
Yamakita,
Y., Ono, S.
& Matsumura, F.(1998).
“Fascin, an actin bundling protein, induces membrane protrusions and increases
cell motility of epithelial cells.” Mol.Biol.Cell
9, 993-1006.
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