| An
RNA-binding protein that binds actin through a C-terminal 15 amino-acid
extension, not present on other Hrp65 isoforms that cannot bind actin (Percipalle
et al, 2003).
The name is derive from "Heterogeneous Nuclear Ribonucleaprotein".
The extension is, and therefore the actin-binding site is:-
PYVNQRPQKARYRNG
Hrp65-2
is a nuclear protein and has been shown (by reversible cross-linking) to
bind actin in the nucleus (Percipalle
et al, 2003).
Both actin (Percipalle
et al, 2001)
and Hrp65-2 (Percipalle
et al, 2003)
are associated with active transcription sites, and interruption of the
complex by introducing competing peptides derived from Hrp65-2 inhibits
transcription. |
| References:-
Percipalle, P., Zhao, J., Pope, B.,
Weeds, A., Lindberg, U. & Daneholt, B. (2001) Actin bound to the
heterogeneous nuclear ribonucleoprotein hrp36 is associated with
Balbiani ring mRNA from the gene to polysomes. J.Cell Biol. 153,
229-236.
Percipalle, P., Fomproix, N., Kylberg,
K., Miralles, F., Bjorkroth, B., Daneholt, B. & Visa, N. (2003) An
actin-ribonucleoprotein interaction is involved in transcription by RNA
polymerase II. PNAS, (in press). |
