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An
actin and microtubule binding protein (Gonzales
et al, 1998).
The protein has been isolated on tubulin affinity columns and when purified then
co-sedimented with F-actin. Analysis of the binding to F-actin suggests an
affinity (Ka) of 1x106M-1.
Immunofluorscence localises Mip-90 to both microtubules and actin in fibroblast
with some nuclear staining also evident. The leading edge where ruffling
membrane underlain by a rich actin network was also stained with Mip-90
antibodies.
References:-
Gonzales, M., Cambiazo,
V., & Maccioni, R.B. (1998). "The interaction of Mip-90 with
microtubules and actin filaments in human fibroblasts. Exp.Cell Res. 239,
243-253.
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