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Previously
known to co-localise with the F-actin cytoskeleton in plants (Cox
& Muday, 1994), NPA was
then found to bind to F-actin columns (Hu
et al, 2000), it
therefore appears that NPA is an actin binding protein, however the possibility
that it binds actin via a third party cannot yet be ruled out.
References:-
Butler,
J. H., Hu, S., Brady, S. R., Dixon, M. W. & Muday, G. K. (1998) In vitro
and in vivo evidence for actin association of the naphthylphthalamic
acid-binding protein from zucchini hypocotyls., Plant J. 13, 291-301.
Cox,
D.N, and Muday, G.K. (1994). "NPA binding activity is peripheral to the
plasma membrane and is associated with the cytoskeleton." Plant Cell
6, 1941-1953.
Hu,
S., Brady, S.R., Kovar, D.R., Staiger, C.J., Clark, G.B., Roux, S.J. and Muday,
G.K. (2000). "Identification of plant actin-binding proteins by F-actin
affinity chromatography." Plant J. 24(1), 127-137.
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