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The
genus Paravahlkampfia has been removed from the genus Vahlkampfia on the basis of
SSUrDNA sequence analysis (Brown & De
Jonckheere, 1999). The original P.
ustiana is described as being 30-65 um in length (Page,
1974), however, a second stain has been
isolated from the gut of lizards that is slightly smaller than this at
between 20-50 um in length (5um width). The cysts of the lizard
strain were also smaller with a diameter of 10-15 um compared to a mean
of 15.4um for the Page strain. This second strain differs in only
4pb in the 1,867 bp of the SSurDNA gene (Schuster
et al, 2003; AJ550994),
and so may be the same species. Other differences between the two
are that the lizard strain cannot feed on E.coli and thrives at
42oC using tissue cultures cells as food. A second species
P. lenta has been isolated from soil near Kelso in Southern Scotland
(Brown & De Jonckheere, 2004).
P. lenta was classified by an analysis of the internal
transcribed spacer including the 5.8S rDNA gene. Mean length 37-80
um and breadth 11-24 um. Locomotion of P. lenta is
sluggish.
Neovahlkampfia is
the nearest genus to
Paravahlkampfia
in some analysis
(Brown & De Jonckheere, 2004)
but not
others (see Heteroboseans on these pages).
Heterolobosea Available
strains:- |
| References:- Brown, S. and J. F. De Jonckheere (1999).
A reevaluation of the amoeba genus Vahlkampfia based on
SSUrDNA sequences. Eur.J.Protistol. 35: 49-54.
Brown, S. & De Jonckheere, J. F.
(2004) Isolation of a new vahlkampfiid amoeba from soil:
Paravahlkampfia lenta n. sp. Eur. J. Protistol. 40,
289-294.
Page, F.
C. (1974) A further taxonomic criteria for limax amoebae, with
descriptions of new species and a key to genera. Arch.Protistenk. 116,
149-184.
Schuster, F. L., De Jonckheere, J. F.,
Moura, H., Spiram, R., Garner, M. M. & Visvesvara, G. S. (2003)
Isolation of a thermotolerant Paravahlkampfia sp. from lizard
intestine: Biology and molecular identification. J.Eukaryot.Microbiol.
50, 373-378.
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