Actin Binding Sequences

Actin Binding Motifs

Small regions of actin binding proteins that have been determined to constitute an actin binding site (Table 1).

Actin Binding Protein	Sequence(s)	References
Actobindin	LKAET
ABP120	VGIGAEELVDKNLKMTLGMIWTIILR	Bresnick et al, 1990, 1991
Annexin	VLIRIMVSR	Jones et al, 1992
Aldolase	ADESTGSIAKRLQSIGTENTE	O'Reilly & Clarke, 1993
ADF/Cofilin	DAIKKK WAPECAPLKSKM	Yonezawa et al, 1989 Yonezawa et al, 1991
CapZ (Dictyostelium b subunit)	NQKYKQLQRELSQVL	Hug et al, 1992
Caldesmon	NLKGAANAEAGSEKLKEKQQEAAVE	Wang et al, 1991
Calponin	AEKQQRRFQPEKLREGRNIIGLQMGTNKFASQQGMTAY	Mezgueldi et al, 1992
CEACAM1	FLHFGKTGSSGPLQ	Schumann et al, 2001
MARKS	KRFSFKKSKLSGFSFKKN	Hartwig et al, 1992
Myosin II head	IRICRKG YRGKKQ EGGGGKKGGKKKGSSF	Vandekerckhove & Vancompernolle, 1992
Myosin II light chain kinase	DFRxxL	Smith et al, 1999
Profilin	GTAANVVEKLADYLIGQGF	Vandekerkhove et al, 1989
Villin ("Head piece")	PAAFSALPRWKQQNLKKEKGLF	Friederich et al, 1992
Misc ABPs	I/LWEQ	McCann & Craig, 1997

Table 1 Actin-Binding Motifs (adapted from Maciver, 1995)

References:-

Bresnick, A., Warren, V. & Condeelis, J. (1990) Identification of a short sequence essential for actin binding by Dictyostelium ABP-120, J. Biol. Chem. 265, 9236-9240.

Bresnick, A. R., Janmey, P. A. & Condeelis, J. (1991) Evidence that a 27-residue sequence is the actin-binding site of ABP-120, J. Biol. Chem. 266, 12989-12993.

Jones, P. G., Moore, G. J. & Waisman, D. M. (1992) A nonapeptide to the putative F-actin binding site of annexin-II tetramer inhibits its calcium-dependent activation of actin filament bundling. J.Biol.Chem. 267, 13993-13997.

Maciver, S. K. (1995) Microfilament organization and actin-binding proteins in The Cytoskeleton (Pryme, H., ed), JAI press inc., Greewich CT, London UK.

McCann, R. O. & Craig, S. W. (1997) The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals., PNAS. 94, 5679-5684.

Schumann, D., Chen, C.-J., Kaplan, B. & Shively, J. E. (2001) Carcinoembryonic antigen cell adhesion molecule 1 directly associates with cytoskeleton proteins actin and tropomyosin., J.Biol.Chem. 276, 47421-47433.

Smith, L., Su, X., Lin, P.-j., Zhi, G. & Stull, J. T. (1999) Identification of a novel actin binding motif in smooth muscle mysoin light chain kinase., J.Biol.Chem. 274, 29433-29438.