|
Although
the Golgi apparatus is best known to be associated with microtubules
(reviewed
Thyberg & Moskalewski, 1999),
actin and many actin binding proteins are known to be associated with the Golgi
body (Valderrama et al, 1998), and disruption of the actin cytoskeleton (for example by C2 Clostridium
botulinum toxin) causes a concomitant collapse of the Golgi (Valderrama
et al, 2000). Some of these ABPs are present as Golgi-specific isoforms
(Table 1), and many (e.g. myosins, filamins) are involved in directional
trafficking whilst others may have a role in structure.
| Actin
Binding Protein |
Function |
Reference |
| Abp1p |
An
SH3 and ADFH containing ABP |
Fucini et al, 2002 |
| ABP280
Filamin |
An
ABP with ability to bind membranes. |
Liu et
al, 1997 |
| Ankyrin |
Not
an ABP directly but anchors spectrin
to the Golgi membrane. Present as a Golgi specific isoform. |
Beck et
al, 1987 |
| Drebrin |
Bundles
microfilaments and is associated with a distinct actin binding
complex |
Fucini
et al, 2000 |
| IQGAP |
A
signalling ABP |
McCallum
et al, 1998;
1999 |
| Profilin
I |
A PIP2
and ABP with signalling involvement |
Dong et
al, 2000 |
| Myosin
II |
Motor
protein |
Stow &
Heimann, 1998 |
| Myosin
VI |
A
minus directed actin based motor
protein |
Buss et
al, 1998 |
| Spectrin |
Membrane
attached ABP that binds many other proteins |
Fath et
al, 1997;
Beck & Nelson, 1998 |
| Tm5 Tropomyosin |
Microfilament stabilization,
regulation. |
Heimann et al, 1999 |
| Table
1 Golgi associated Actin Binding Proteins
|
ADP-ribosylation
factors and Golgi regulation.
ADP-ribosylation factors (ARFs) regulated vesicle transport (Mellman
& Warren, 2000;
Fucini et al, 2000)
and actin. ARF regulates the association of spectrin with the Golgi
membrane (Godi et
al, 1998), and
the association of actin to the Golgi via Rho family G-protein
localization (Rac, CDC42, but not Rho itself) (Erickson
et al, 1996;Fucini
et al, 2002;Wu
et al, 2000)
and WASP/Arp2/3 complex (Fucini
et al, 2002).
 The
Golgi Apparatus
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