| Originally
identified in yeast, as an actin binding protein (Drubin
et al, 1990)
associated with the membrane (Mulholland
et al, 1994),
Abp1p has an ADF homology domain and an SH3 domain. Mammalian
homologs have been discovered and are thought to have a role in
endocytosis (Kessels
et al, 2000 & 2001).
Although not an essential gene in itself (Holtzman
et al, 1993; Adams et al, 193),
Abp1p is required in fimbrin nulls and SLA1 SLA2 nulls. Abp1p binds
actin through a region homologous to ADF/cofilin and to dynamin through
the SH3 domain (Kessels
et al, 2001).
It is possibly through the interaction of dynamin that Abp1p may have a
function to play in the complex process of endocytosis (Jeng
& Welsch, 2001).
It is surprising that Abp1p is not an essential gene (in yeast) as this
protein seems to be involved in so many different many actin -dependent
activities.
A number of
binding partners for the SH3 domain and other domains have now been
established (Table 1).
| Protein |
Function |
Abp1p
domain bound |
References |
| Actin |
Forms
filaments |
ADH
domain |
|
| Ark1p |
Actin
regulating kinase |
SH3 |
Fazi
et al, 2002 |
| Arp2/3
complex |
Nucleates
actin filaments and caps pointed ends of filaments. |
|
Goode
et al, 2001;
Evangelista, et
al, 2000 |
| Myosin
V |
Actin
based motor |
SH3 |
Fazi
et al, 2002 |
| Prk1p |
Actin
regulating kinase |
SH3 |
Fazi
et al, 2002 |
| RVS167 |
|
SH3 |
Lila
& Drubin, 1997 |
| Srv2p
CAP |
An
adenylyl cyclase associated protein that activates its activity
which itself is an actin-binding protein. |
SH3 |
Lila
& Drubin, 1997 |
| Synaptojanin |
|
SH3 |
Fazi
et al, 2002 |
| |
|
|
|
| Table
1 |
|
|
|
References:-
Adams, A. E. M., Cooper, J. A. &
Drubin, D. G. (1993) Unexpected combinations of null mutations in genes
encoding the actin cytoskeleton are lethal in yeast, Mol.Biol.Cell. 4,
459-468.
Drubin, D. G., Mulholland, J., Zhu, Z.
& Botstein, D. (1990) Homology of a yeast actin-binding protein to
signal transduction proteins and myosin-I, Nature. 343,
288-290.
Evangelista, M.
Fazi, B., Cope, M. J. T. V.,
Douangamathi, A., Ferracuti, S., Schirwitz, K., Zucconi, A., Drubin, D.
G., Wilmanns, M., Cesareni, G. & Castagnoli, L. (2002) Unusual
binding properties of the SH3 domain of the yeast actin-binding protein
Abp1., J.Biol.Chem. 277, 5290-5298.
Goode, B. L., Rodal, A. A., Barnes, G.
& Drubin, D. G. (2001) Activation of the Arp2/3 complex by the actin
filament binding protein Abp1p., JCB. 153, 627-634.
Holtzman, D. A., Yang, S. & Drubin,
D. G. (1993) Synthetic-lethal interactions identify two novel genes,
SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces
cerevisiae, J.Cell Biol. 122, 635-644.
Jeng, R.L. & Welsh, M.D. (2001)
Cytoskeleton: Actin and endocytosis- no longer the weakest link. Current
Biology 11, R691-R694
Kessels, M. M., Engqvist-Goldstein, A.
E. Y., Drubin, D. G. & Qualmann, B. (2001) Mammalian Abp1, a
signal-responsive F-actin-binding protein, links the actin cytoskeleton
to endocytosis via the GTPase dynamin., J.Cell Biol. 153,
351-366.
Kessels, M. M., Engqvist-Goldstein, A. E. Y.
& Drubin, D. G. (2000) Association of mouse actin-binding protein 1
(mAbp1/SH3P7), an Src Kinase target, with dynamic regions of the
cortical actin cytoskeleton in response to Rac1 activation., Mol.Biol.Cell.
11, 393-412.
Lila, T. & Drubin, D.G. (1997). Evidence
for physical and functional interactions amongst two Saccharomyces
cerevisiae SH3 domain proteins, an adenylyl cyclase-associated
protein and the actin cytoskeleton Mol.Biol.Cell 8,
367-385.
Mulholland, J., Preuss, D., Moon, A.,
Wong, A., Drubin, D. & Botstein, D. (1994) Ultrastructure of the
yeast actin cytoskeleton and its association with the plasma membrane, J
Cell Biol. 125, 381-91.
Olazabal, I. M. & Machesky, L. M.
(2001) Abp1p and cortactin, new "hand holds" for actin., J.Cell
Biol. 154, 679-682.
Yang, H.-C. & Pon, L. A.
(2002) Actin cable dynamics in budding yeast., PNAS. 99, 751-756.
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