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A
17 kDa actin-binding protein originally isolated from Dictyostelium (de
Hostos et al, 1993)
but since found in humans where it has been discovered to be a binding partner
for 5-Lipoxygenase (5LO) (Provost
et al, 2001)
an enzyme pivotally involved in the production of leukotrienes from arachidonic
acid. 5LO is itself an actin-binding protein (Lepley
& Fitzpatrick, 1994;
Provost et al,
2001a). The
human version of coactosin is named CLP (Coactosin-Like Protein), an F-actin but
not a G-actin binding protein (Provost
et al 2001b)
that apparently inhibits the capping activity of CapZ , despite the fact that
CLP itself does not alter the kinetics of actin polymerization (Rohrig
et al, 1995).
CLP is expressed in lung, placenta, kidney and leucocytes (Provost
et al 2001b)
co-localizing with fibers in transfected COS cells.
Coactosin and CLP both
show homology to the ADF/cofilin family (de
Hostos et al, 1993)
and yet it contains a LKKAET -like motif found in other actin-binding proteins
that in the case of CLP is responsible for binding 5LO (Provost et al,
2001a).
Lysine 75 has been found to be important for the binding of actin (Provost
et al 2001b).
References
de Hostas, E. L., B. Bradtke, et al.
(1993). “Coactosin, a 17 kDa F-actin binding protein from Dictyostelium
discoideum.” Cell
Mot.Cytoskeleton 26, 181-191.
Lepley, R.A. &
Fitzpatrick, F.A. (1994). J.Biol.Chem. 269, 24163-24168
Provost, P., Doucet, J., Hammarberg, T.,
Gerisch, G., Samuelsson, B. & Radmark, O. (2001a) 5-Lipoxygenase interacts
with coactosin-like protein., J. Biol.Chem. 276, 16520-16527.
Provost, P., Doucet, J., Stock, A., Gerisch,
G., Samuelsson, B. & Radmark, O. (2001b) Coactosin-like protein, a human
F-actin-bining protein: critical role of lysine-75., Biochem. J. 359,
255-263.
Rohrig, U., Gerisch, G., Morozova, L.,
Schliecher, M. & Wegner, A. (1995) Coactosin interferes with the capping of
actin filaments., FEBS letters. 374, 284-286.
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