|
A
23kDa Calponin-related protein
(Shapland et al,
1988; Prinjha
et al, 1994) found in smooth muscle and non-muscle cells. The protein
binds actin in vitro only if the salt concentration is low, however
transgelin co-localises with F-actin in cells by immunofluorescence microscopy (Shapland
et al, 1988),
indicating that
perhaps another factor influences actin binding in vivo. The
expression of this protein is transformation sensitive and is lost in a variety
of cell types upon transformation, possibly through the deregulation of the Ras
pathway. However, no decrease in the transformed phenotype was seen as the
transgelin was re-expressed in the transformed cells (Shields
et al, 2002).
This suggests that transgelin itself is not vitally important in the
transformation process but is perhaps a "side show".
References:-
Lawson,
D., Harrison, M., and Shapland, C. (1997). Cell Mot.Cytoskel. 38,
250-257.
Prinjha, R. K., Shapland, C. E., Hsuan, J. J.,
Totty, N. F., Mason, I. J. & Lawson, D. (1994) Cloning and
sequencing of cDNAs encoding the actin cross-linking protein transgelin
defines a new family of actin-associated proteins, Cell Motility and
the Cytoskeleton. 28, 243-255.
Shapland, C., Lowings, P. &
Lawson, D. (1988) Identification of new actin-associated polypeptides
that are modified by viral transformation and changes in cell shape. J.Cell
Biol. 107, 153-161.
Shapland, C., Hsuan, J. J., Totty, N. F. &
Lawson, D. (1993) Purification and properties of transgelin: A
transformation and shape change sensitive actin-gelling protein., J.Cell
Biol. 121, 1065-1073.
Shields, J. M., Roger-Graham, K. &
Der, C. J. (2002) Loss of transgelin in breast and colon tumors and in
RIE-1 cells by Ras deregulation of gene expression through Raf-independent
pathways. J.Biol.Chem. 277, 9790-9799. |
